NSF

Corona virus - COVID-19 - 25/06/2020
All our activities and services are back to normal, but please note that only in the week of 28 (6/7-10/7) no NGS activities will take place in NSF. Sanger sequencing will remain operational.

For any questions please contact us via email (gsf@uantwerpen.be) or on tel. number: 03 2651108. We apologize of any inconvenience that might cause but wellbeing and safety is our priority. Thank you for understanding,

NSF team

NSF

Neuromics Support Facility (NSF) is a competence center providing services and training in the fields of genetics, genomics, transcriptomics, bioinformatics, histology and cellular imaging. Our vision is to maintain state-of- the-art, reliable, efficient and flexible support to the main research activities of the VIB-UAntwerp Center for Molecular Neurology (CMN).

Our responsibilities include evaluation of the novel scientific and commercial tools and technologies, consultations, user training and experiment design support in all areas of our proficiency. Neuromics Support Facility is led by Dr. Mojca Strazisar and includes three technological and expertize sectors.

Services

Sequencing

Genomic Service Facility utilizes Applied Biosystems® and Illumina technologies and provides high- throughput Sanger, targeted, exome, transcriptome sequencing, genotyping and fragment analysis. We perform also a variety of auxiliary services, including library preparation (exome, genome, transcriptome), sample quality control (concentration, sizing, purity, degradation) and sample manipulation (short and long fragmentation, dilution, aliquoting, pooling, and purification). All activities are fully or partially automated, governed by good laboratory practice and documented using a digitalized environment (LIMS and ELN).

Microscopy

We offer access to basic and advanced equipment for light, fluorescence and confocal microscopy. Optimal imaging and analysis protocols are tailored to the biological questions related to the complex neurological diseases of the central and peripheral nervous system. The use of quantitative imaging assays provides a valuable tool to analyze and compare mutation induced complex biological disturbances in cells and model organisms. Services extend to the management and execution of the experiments (imaging and analysis) and establishing networks to access external expertise and infrastructure.

Bioinformatics

Our bioinformatics expertize sector manages, implements, executes and maintains data analysis and storage on the hardware and software level. Main focus of the analysis support lies in providing solutions using customised, comprehensive evaluation of genomic and transcriptomic data and includes current, advanced gene set and pathway modelling and annotations. Novel protocols and pipelines are developed to enhance the primary data analysis, which is complemented by different workflows and in- house created or optimized tools to enhance the interpretation of the results.

Sanger Sequencing

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